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Efficiency of HIV-1 PR-RT genotyping is not impacted by co-infection with HCV

Background

Assays that are used for the diagnosis and management of HIV-1 infection are subject to assay interference(s) and co-infection with HCV may interfere with HIV-1 genotyping and/or phenotyping. Since a third of HIV-1 infected patients are frequently HCV co-infected, there is an increasing need to assess the effect of HCV co-infection on the accuracy of the HIV-1 protease (PR) and reverse transcriptase (RT) genotyping.

Purpose of the study

As a result, the efficiency and accuracy of Virco's HIV-1 PR-RT assay performance [1] was tested on a panel of HIV/HCV co-infected, clinical trial samples.

Materials and methods

A panel of confirmed 60 HIV/HCV-positive (Hep C antibody +), plasma samples (screening visit), that were collected as part of a HIV-1 clinical trial was PR-RT genotyped (virco®TYPE HIV 1; 1497 bp encoding 1-99 amino acids of PR and 1-400 amino acids of RT), phenotyped (Antivirogram®) and the Clade was determined. In addition, HCV subtyping was performed using NS5B sequence-based subtyping [2] along with NS3/4A genotyping [3] in all of the tested samples. All the 60 samples tested had externally determined plasma HIV viral loads that were >1000 copies/mL.

Results

For the 60 HIV/HCV + samples tested, the HIV PR-RT target genes were successfully (100%) genotyped, phenotyped and were confirmed to be Clade B. Subtyping (329 bp conserved fragment within NS5B) and genotyping (NS3/4A gene) of the HCV target genes was successful for 49/60 samples (81.6%) and 33 samples were identified as Genotype 1a, 10 samples as 1b, 5 samples as 2b and 1 as genotype 4a.

Conclusions

We demonstrated that there is no assay interference for HIV-1 genotyping/phenotyping in the presence of an active HCV co-infection. The HIV-1 PR-RT primers used within our certified, high-throughput laboratory is highly sensitive, specific, accurate and reliable to detect HIV-1 clade, genotype and phenotype in HIV/HCV co-infected samples.

References

  1. Van Houtte , et al: J. Med Vir. 2009, 81 (10): 1702-9. 10.1002/jmv.21585.

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  2. Koletzki , et al: Clin Chem Lab Med. 2010, 48 (8): in press

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  3. Dumont , et al: Poster number 319. 2009, EASL

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This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Deloof, A., De Wolf, H., Verlinden, Y. et al. Efficiency of HIV-1 PR-RT genotyping is not impacted by co-infection with HCV. JIAS 13, P212 (2010). https://doi.org/10.1186/1758-2652-13-S4-P212

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  • DOI: https://doi.org/10.1186/1758-2652-13-S4-P212

Keywords

  • Screen Visit
  • Assay Performance
  • Trial Sample
  • Assay Interference
  • Clinical Trial Sample