Table 1 Criteria for laboratory evaluation
From: The Brazilian Network for HIV-1 Genotyping External Quality Control Assurance Programme
Sample 1 | Sample 2 | |||
|---|---|---|---|---|
Qualitative variables | ||||
Positive PCR amplification | ||||
15 | 15 | 30 | ||
Genetic diversity of sequenced samples | ||||
Less than 2.2% | 10 | 10 | 20 | |
Quantitative variables | ||||
Number of obtained sequences | ||||
4 | 2 | 2 | ||
5 | 4 | 4 | ||
6 | 8 | 8 | ||
7 | 10 | 10 | 20 | |
Correct interpretation of mutations (codons) | ||||
Protease 1 | 1 | 1 | ||
2 | 1 | 1 | ||
3 | 1 | 1 | ||
4 | 1 | 1 | ||
5 | 1 | 1 | 10 | |
Reverse transcriptase 1 | 1 | 1 | ||
2 | 1 | 1 | ||
3 | 1 | 1 | ||
4 | 1 | 1 | ||
5 | 1 | 1 | 10 | |
Quality of sequences (Phred quality scores) | ||||
100% above 30 | 5 | 5 | ||
80-99% above 30 | 2.5 | 2.5 | 10 | |
Total | 100 | |||