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  • Oral presentation
  • Open Access

Myeloid dendritic cells induce HIV-1 latency in non-proliferating CD4+ T cells

Journal of the International AIDS Society201013 (Suppl 3) :O7

  • Published:


  • Lymphoid Tissue
  • Latent Infection
  • Ki67 Expression
  • Productive Infection
  • Myeloid Dendritic Cell


Resting CD4+ T cells within lymphoid tissues are a reservoir of latent infection; however, in isolated resting CD4+ T cells, several blocks exist that restrict HIV-1 replication. We hypothesize that interactions with dendritic cells (DCs) within lymphoid tissues contribute to the establishment of latency.


SNARF-labelled resting CD4+ T cells were cultured alone or with DC for 24 prior to mock infection or infection with a CCR5-using, EGFP-reporter virus. Non-proliferating (SNARFhi) CD4+ T cells that were not productively infected (EGFP-) were purified five days post infection and: (1) latent infection was reactivated and amplified by co-culturing the sorted cells with mitogen-stimulated PBMC for five days; and (2) gene expression changes were compared in sorted non-proliferating CD4+ T cells cultured in the presence or absence of DCs with or without HIV-1 infection using oligonucleotide microarrays.


In the presence of DCs, a significant increase in the number of latently infected non-proliferating CD4+ T cells (p=0.01) was observed when compared with resting CD4+ T cells cultured alone. These cells had not entered into the cell cycle as confirmed by the lack of Ki67 expression, although 2% of the DC co-cultured cells did express the early activation marker CD69. Post-integration latency was detected in the non-proliferating CD4+ T cells following co-culture with sorted myeloid (mDC) but not plasmacytoid DC (pDC), which was confirmed using Alu-LTR PCR to detect integrated HIV-1 DNA (11,000 and <300 copies/million cells, respectively). We identified 193 genes that were differentially expressed in the latently infected non-proliferating CD4+ T cells. Observations include the induction of multiple genes associated with cell cycle arrest and the inhibition of HIV-1 transcription.


Our results suggest a possible pathway for mDC-induced latency in CD4+ T cells in which low levels of cell activation may allow for enhanced HIV-1 integration, but subsequent blocks in transcription and cell proliferation prevent progression to productive infection.

Authors’ Affiliations

Department of Medicine, Monash University, Victoria, Australia
CHUM-Research Center, Saint-Luc Hospital, Montreal, Canada
VGTI-Florida, Port St Lucie, USA
Infectious Diseases Unit, Alfred Hospital, Melbourne, Australia
Burnet Institute, Melbourne, Australia