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  • Oral presentation
  • Open Access

Novel pathways of transcriptional and post-transcriptional regulation of post-integrative HIV-1 latency

Journal of the International AIDS Society201013(Suppl 3):O4

https://doi.org/10.1186/1758-2652-13-S3-O4

Published: 04 November 2010

Keywords

Cellular FactorViral Gene ExpressionViral ReservoirSilent StateChromatin Conformation

Background

Therapeutic targeting of HIV latency requires an understanding of the basic mechanisms regulating viral quiescence and activation.

Methods

We identified several levels of transcriptional and post-transcriptional control of viral genes expression. By looking at the organization of chromatin at the site of integration by chromatin conformation capture and in situ hybridization, we found that chromatin imposes silencing in a highly specific spatial and temporal pattern. We also identified several cellular factors that interact with viral RNA by a proteomic approach, pointing to post-transcriptional processes that have been greatly overlooked as mechanisms of HIV post-integrative latency.

Results

HIV-1 can integrate within active genes at the periphery of the nucleus, and silencing may involve repression from a peri-centromeric region in trans. Hence, a mechanism of active spatial reorganization of chromatin at the site of proviral integration may be ultimately responsible for virus silencing. Particularly relevant to flushing therapies of the viral reservoir is the observation that the silent state of an integrated provirus in a clonal population of activated lymphocytes is not homogeneous, with a small number of cells carrying a provirus embedded into heterochromatin, compared with the majority of cells where the provirus is already poised for transcription.

Proteomic analysis revealed several novel cellular factors, including PSF, MATR3 and p54nrb that have been previously in nuclear retention of RNA. We demonstrated that PSF/p54nrb binds nascent HIV RNA at the transcription site, but MATR3 defines a novel sub-nuclear compartment where this viral RNA is retained. Rev is able to associate with unspliced HIV RNA co-transcriptionally directing its nuclear export. These observations lead to a model for a novel cellular pathway of RNA retention that is hijacked by the virus.

Conclusions

Both the control imposed by the organization of the nucleus and by post-transcriptional mechanisms is relevant for the control of HIV-1 post-integrative latency.

Authors’ Affiliations

(1)
International Centre for Genetic Engineering and Biotechnology, Trieste, Italy

Copyright

© Marcello et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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