Table 2 Overall performance of laboratories according to each evaluated criterion as a percentage of the maximum score
From: The Brazilian Network for HIV-1 Genotyping External Quality Control Assurance Programme
Sample 1 | Sample 2 | |||
|---|---|---|---|---|
Qualitative variables | ||||
Positive PCR amplification | ||||
90.5% | 92.9% | 91.7% | ||
Genetic diversity of sequenced samples | ||||
Less than 2.2% | 100% | 100% | 100% | |
Quantitative variables | ||||
Number of obtained sequences | ||||
4 | 0% | 0% | ||
5 | 6.0% | 4.8% | ||
6 | 6.0% | 3.6% | ||
7 | 75.9% | 80.7% | 88.6% | |
Correct interpretation of mutations (codons) | ||||
Protease | 1 | 0% | 0% | |
2 | 1.2% | 1.2% | ||
3 | 0% | 0% | ||
4 | 1.2% | 1.2% | ||
5 | 85.7% | 88.1% | 89.3% | |
Reverse transcriptase | 1 | 0% | 0% | |
2 | 1.2% | 1.2% | ||
3 | 0% | 0% | ||
4 | 1.2% | 1.2% | ||
5 | 85.7% | 88.1% | 89.3% | |
Quality of sequences (Phred quality scores) | ||||
100% above 30 | 22.7% | 25.0% | 23.8% | |