Volume 13 Supplement 4

Abstracts of the Tenth International Congress on Drug Therapy in HIV Infection

Open Access

Transmitted drug resistance associated with transmission clusters in newly diagnosed antiretroviral-naïve patients in Northern Greece

  • L Skoura1,
  • S Metallidis2,
  • AJ Buckton3,
  • JL Mbisa3,
  • D Pilalas2,
  • E Papadimitriou1,
  • A Papoutsi1,
  • AB Haidich4,
  • D Valagouti2,
  • O Tsachouridou2,
  • ZA Antoniadou1,
  • P Kollaras2,
  • P Nikolaidis2 and
  • N Malisiovas1
Journal of the International AIDS Society201013(Suppl 4):P125

DOI: 10.1186/1758-2652-13-S4-P125

Published: 8 November 2010

Purpose of the study

To determine the contribution of transmission clusters on transmitted drug resistance (TDR) in newly diagnosed antiretroviral naive patients in Northern Greece, during 2000 -2007.

Methods

Viral reverse transcriptase and protease genes from 369 individuals with newly diagnosed HIV-1 infection were sequenced at baseline. A maximum-likelihood phylogenetic analysis method was employed to examine for linkage between viral isolates. Clinical data were retrieved from the database and cross-referenced with the patients' medical files. Transmitted drug resistance was defined in accordance with the Surveillance Drug Resistance Mutation (SDRM) 2009 list.

Results

The study population characteristics were as follows: 82.8% male, 89.7% of Greek nationality, mean age 38, median CD4 cell count at diagnosis 295 cells/µl and mean HIV-1 RNA 4.94 log10 copies/ml. The most prevalent risk exposure category was men who have sex with men 59.1% (n=218) followed by heterosexual transmission 21.4% (n=79) and intravenous drug use 7.6% (n=28). Subtype B viruses were most prevalent (53.1%), followed by subtype A (32.5%). At least one drug resistance mutation was identified in 46/369 patients (12.4%). Twenty-eight patients (7.6%) harbored resistance mutations to nucleoside/nucleotide RT inhibitors, 20 patients (5.4%) to non-nucleoside RT inhibitors and 12 patients (3.3%) to protease inhibitors (PIs). Dual-class resistance mutations were identified in 14 patients (3.8%). The median CD4 cell count in patients with TDR was not significantly different compared to patients without (p=0.072). Phylogenetic analyses, supported by bootstrapping >90% and genetic distance <0.015, revealed three transmission clusters involving drug resistant strains, including one cluster of 11 patients infected with a strain carrying RT mutations Y181C and T215 variants conferring NRTI and NNRTI resistance.

Conclusions

The overall prevalence of TDR in our study population was 12.4%. Phylogenetic analyses of viral sequences from these new diagnoses demonstrated the impact of transmission clusters on the primary drug resistance. The outbreak of dual-class TDR, coupled with late HIV diagnosis in this population may require improved public health interventions.

Authors’ Affiliations

(1)
Medical School, Aristotle University of Thessaloniki, National AIDS Reference Center of Northern Greece
(2)
Infectious Diseases Division, AHEPA University Hospital, Medical School, Aristotle University of Thessaloniki
(3)
Colindale, Virus Reference Department, Centre for Infections, Health Protection Agency
(4)
Medical School, Department of Hygiene, Aristotle University of Thessaloniki

References

  1. Bennett DE, Camacho RJ, Otelea D, et al: Drug Resistance Mutations for Surveillance of Transmitted HIV-1 Drug-Resistance. PLOS ON 2009, 4: e4724, 2009 Update.View ArticleGoogle Scholar

Copyright

© Skoura et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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